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Related websites
SYBR Green I - Wikipedia
WEBsybr green I (SG) is an asymmetrical cyanine dye [1] used as a nucleic acid stain in molecular biology. The SYBR family of dyes is produced by Molecular Probes Inc., now owned by Thermo Fisher Scientific. sybr green I binds to DNA. The resulting DNA-dye-complex best absorbs 497 nanometer blue light (λ max = 497 nm) and emits green light …
En.wikipedia.orgSYBR™ Green I Nucleic Acid Gel Stain, 10,000X concentrate in …
WEBSYBR™ Green I Nucleic Acid Gel Stain is one of the most sensitive stains available for detecting double-stranded DNA (dsDNA) in agarose and polyacrylamide gels. Because SYBR™ Green I has greater sensitivity for dsDNA, it is especially useful for assays where the presence of contaminating RNA or ssDNA might obscure results.
Thermofisher.comSYBR Green I - an overview | ScienceDirect Topics
WEBsybr green I is a fluorescent dye that preferentially binds double-stranded DNA (dsDNA) in a sequence-independent manner. When sybr green I dye binds to dsDNA, the fluorescence emission level of sybr green I increases considerably.
Sciencedirect.comSYBR® Green | Bio-Rad
WEBSYBR ® Green real-time PCR assays are ideally suited for high-throughput screening programs designed to detect single targets in many samples, with applications in applications in clinical research, gene expression studies, population genetics, and environmental and food safety testing.
Bio-rad.comSYBR® Green for qPCR | Bio-Rad
WEBSYBR ® Green is a dsDNA-binding dye that intercalates nonspecifically into dsDNA, allowing measurement of the amount of PCR product. SYBR ® Green fluorescence increases up to 1,000-fold upon intercalation with dsDNA.
Bio-rad.comSYBR® Green Master Mix | Bio-Rad
WEBUse sybr green master mix for reliable performance with all templates on any real-time PCR instrument. Cost-effective, sequence-independent qPCR results.
Bio-rad.comHow does qPCR work: SYBR Green vs TaqMan | INTEGRA
WEBMay 11, 2022 · qPCR relies on fluorescence from intercalating dyes or hydrolysis probes to measure DNA amplification after each thermal cycle. The most common dye-based method is sybr green, and the most common probe-based method is TaqMan, which is why this article will focus on these two qPCR techniques.
Integra-biosciences.comSYBR Green I - an overview | ScienceDirect Topics
WEBsybr green I is a dye that binds to double-stranded DNA during the PCR process and increases its fluorescence, allowing for the quantification of the amount of amplicon produced. However, it has the drawback of non-specific binding and can result in signal saturation with longer amplicons.
Sciencedirect.comComparison of SYBR Green and TaqMan methods in quantitative …
WEBFeb 28, 2014 · In this study, we compared two common RT PCR methods, TaqMan and sybr green in measurement gene expression profile of adenosine receptors. Gene expression profiles of A1, A2A, A2B and A3 Adenosine receptors were analyzed by optimized TaqMan and sybr green quantitative RT PCR in breast cancer tissues.
Ncbi.nlm.nih.govComparison of SARS-CoV-2 indirect and direct RT-qPCR …
WEBMay 17, 2021 · For RNA detection, we tested TaqMan-based detection systems from BGI and Norgen Biotek, as well as a sybr green method using a commercially available RT-qPCR mix and published primers (some used for sybr green and others from probe-based methods) along with new primers we developed.
Ncbi.nlm.nih.gov